1.  Experiments:  Mitochondrial Respiration and Photosynthesis
     in Isolated Thylakoids

In this month's newsletter, two experiments that utilize a dissolved oxygen electrode and a cellular respiration/photosynthesis chamber are featured. 

In the mitochondrial respiration experiment, the amount and rate of oxygen consumed by mitochondria in the presence of different combinations of substrates, inhibitors, and uncouplers indicates the flow of electrons through the molecules that are part of the electron transport chain (ETC).  The energy released as electrons pass through the ETC is used to synthesize ATP. 

In the photosynthesis experiment, the amount and rate of oxygen produced by chloroplasts indicates the light-driven flow of electrons through the photosystems that convert light energy to chemical energy.  The energy captured is used to synthesize sugars from carbon dioxide and water.

In these experiments, the students use  the ISE-730 dissolved oxygen electrode, the DO2-100 current to voltage adapter that converts the output of the ISE-730 to a measurable voltage, and the RPC-100 respiration/photosynthesis chamber.
 

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mitochondrial experiment (PDF file).

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photosynthesis experiment (PDF file).

Download high resolution, press optimized mitochondrial experiment PDF file. 

Download low resolution, screen optimized mitochondrial experiment PDF file.

Download high resolution, press optimized photsynthesis experiment PDF file. 

Download low resolution, screen optimized photosythesis experiment PDF file.

2.  Articles:  The RPC-100 Respiration/Photosynthesis Chamber,
    the ISE-730 Oxygen Electrode, and the DO2-100 Current to
    Voltage Adapter.

The RPC-100 is a reaction chamber designed for studying cellular respiration or photosynthesis.  The chamber is filled with a mixture of cells or cell fractions, buffers, and reagents that react to either consume or produce oxygen.  The amounts and rates of oxygen, consumed or produced, are measured using an ISE-730 oxygen electrode placed in the chamber.  The current output of the oxygen electrode is converted to a voltage output by the DO2-100 adapter which also amplifies the output voltage before it is recorded.  

Learn more about the RPC-100 from this tech note.  Learn more about the ISE-730 and the DO2-100 from this tech note.

Special Offer to iWorx Users  
For a limited time, iWorx is offering its customers a 30% discount on purchase of the RPC-100 used in the experiment featured above.

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3. Download: Settings for the Experiments Highlighted This
    Month and the Other Experiments Recently Published in the
    iWorx Newsletter

If you plan to use any of the experiments or manuals announced in recent editions of the iWorx Newsletter, you should obtain a copy of the settings group used to configure LabScribe for these experiments.  Click here to download copies of the settings groups for the experiments or manuals announced since May 2004.  Once you have a copy of the settings group, just drop the group into the LabScribe folder on your hard drive, and use the Settings menu to load the group and a list of its experiments.
 

Download copies of the settings groups for the experiments and manuals announced since May 2004. 

4.  Web Resources

Additional information on this month's experiments can be found under Cellular Respiration and Photosynthesis here.

On this page we have also accumulated a host of links to informative, external physiology Web sites and conveniently categorized them into major sub categories for easy reference.   

5.  LabsOnCD - A Requirement for Distance Learning
     Physiology Curriculum

Each lab includes a topical overview, background, equipment set-up and lab procedure, quiz questions, and an interactive LabPlayer that displays recorded data from real experiments and allows students to manipulate and record data just as they would in a "wet" lab.  Data can be captured and logged in an online Lab Journal that can be later copied to a new document or sent via email.  Labs are further enhanced with animations, diagrams, and video clips. 

The virtual labs can be accessed on a CD or from the Web and are designed specifically for student self-learning environments.

See more information, view an online demonstration, and request a free 30-day evaluation copy.

6.  Trade-in Specials