Various physiological parameters can be measured in rodents such as mice, rats, squirrels, prairie dogs, porcupines, beavers, guinea pigs, hamsters, rabbits, hares, etc…
ECG signals can be measured with the IX-BIOx. Measuring 2 channels of ECG, Lead I and Lead II allows LabScribe software to calculate Lead III, aVR, aVF and aVL . EMG signals can be measured at the same time as the ECG signals.
iWorx LabScribe ECG Software Module is a powerful and user-friendly tool intended to automate the analysis of ECG data from a variety of species.
Features:
Measure R-R, PR, QT, QR and QTc intervals, QRS, T, P and TP durations, P, Q, R, S, T amplitudes and ST elevation, etc...
Specific analysis templates are included to accurately delineate PQRST onset, durations and amplitudes
Custom templates can also be created based on unique ECG profiles and saved to a library for future use
Beat averaging and classification
Outlier removal based on abnormal R-R intervals, heart rate, noise and activity
Easily extract source data and average data as images or text files
Plug and play compatible with all iWorx data acquisition
Optional ASCII Text Import Module is also available that enables import of ECG data in text or edf format that was recorded by any other acquisition program
Heart Rate Variability
Kubios HRV - Heart Rate Variability Analysis Software is an advanced tool for studying the variability of heart beat intervals. Data can be exported from LabScribe using the edf export option and then imported into Kubios HRV for analysis, as shown in this technote
The iWorx ASCII Import Module enables import of data in text format that was recorded in any other data acquisition program. The module also allows the researcher to use the analysis functions in LabScribe software on data they may have previously recorded with a different program.Detailed information about using the modules is in the LabScribe Manual
iWorx systems, recorders and software have been used for recording ERGs in rodents and insects as noted in these Citations.
Amplifiers
Differential Amplifier (DP-301) by Warner Instruments The DP-301 is a single channel AC/DC differential pre-amplifier highly suited for EEG, EKG and extracellular recording.
Differential Amplifier (Model 3000) by A-M Systems The Model 3000 High-Gain AC/DC Differential Amplifier is designed for low-noise recordings from excitable tissue. Specific applications include EEG, EKG, extracelluar recordings, and more.
Recorders
iWorx IX-RA-834 8 Channel Recorder The iWorx RA-834 Recorder is the most advanced and feature-rich physiological data acquisition system on the market.
iWorx IX-400 Series Recorders The iWorx 400 Series recorders are economical, high performance four, eight or sixteen channel data recorders for use with a broad range of analog amplifiers.
IBPProper assessment of cardiac function and hemodynamic performance requires a measurement of pressure from within the heart and peripheral arteries. iWorx offers several solutions for measuring pressure including fluid‐filled pressure transducers, catheter-tipped micro pressure sensors and non‐invasive tail cuff pressure systems.LabScribe software with the Blood Pressure Module provides built‐in software routines to perform a number of arterial and ventricular pressure calculations
Fluid-Filled Catheter System
The BP-100/102 blood pressure transducer is an economical and accurate device used to measure direct arterial or venous pressure in animals using the fluid-filled catheter technique. A precision laser-trimmed chip provides accurate and linear measurements over a broad range of pressures. Pressure changes in the vessel are transmitted within the fluid-filled tube to the pressure transducer and acquired for monitoring and further analysis. Tubing is available as small as 1 mm in outside diameter making this technique well-suited for measuring pressures in very small vessels. Blood pressure systems include the transducer, cable, data recorder and software.
Bladder Pressure
The BP-100/102 blood pressure transducer can also be used to measure bladder pressure.
BP-100 Intravascular Blood Pressure ProbeMeasures direct arterial or venous pressure in animals.BP-100RE Replacement Element for BP-100 Probe
BP-102 Blood Pressure TransducerWith integral 3cc flush device and stopcock, can be used to measuredirect arterial or venous pressure in animals.
Recorders
IX-RA-834 RecorderThe iWorx RA Recorder is the foundation for iWorx advanced research systems. It include 4 transducer inputs, for recording 4 channels of Blood Pressure. Additional 4 channels of Blood Pressure can be recorded using the IA-400D amplifier and the 4 BNC inputs of the IX-RA-834. IX-RA-834 provides the ease of use and flexibility to speed your research.
IX-404 RecorderThe iWorx 404 Recorder is an economical, high performance four channeldata recorders for use with a broad range of analog amplifiers. Therecorder offers 16-bit resolution at a maximum data collection speed of10 kHz, making it appropriate for most research applications.
Amplifier
IA-400DDedicated bridge amplifier for iWorx-brand transducers that providesfour channels of excitation power, gain, and noise filtration.
LS-BP The LabScribe Blood Pressure Module acquires and analyzes data from blood pressure transducers. Data can be analyzed from ventricular and arterial signals in real-time. The software automatically calculates common indices of function from the blood pressure signal where it can easily be exported to another program for further analysis.Arterial Pressure Calculations:
Heart Rate (HR)
Maximum Pressure (Pmax)
Minimum Pressure (Pmin)
Mean Arterial Pressure (MAP)
Systolic and diastolic pressure
Maximum dP/dt (dP/dtmax)
Minimum dP/dt (dP/dtmin)
Pulse Height
Notch Pressure
Systolic Duration
Diastolic Duration
Cycle Duration
Time To Peak
Election Time
Percent Recovery
Ventricular Pressure Calculations:
Heart Rate (HR)
Maximum Pressure (Pmax)
Minimum Pressure (Pmin)
Mean Pressure (Pmean)
End-systolic Pressure (Pes)
End-diastolic Pressure (Ped)
Maximum dP/dt (dP/dtmax)
Minimum dP/dt (dP/dtmin)
Developed Pressure - dP/dt@
Contractility Index
Relaxation Index
Systolic Duration
Diastolic Duration
Cycle Duration
Tension Time Index
Relaxation Time
Isovolumetric Time
Tau (Weiss, Logistic, Glantz, Mirsky)
Detailed information about using the modules is in the LabScribe Manual
NIBP
Non-Invasive Blood Pressure Systems
Invasive measures of pressure are not always possible and, as a result, there may be a need for non-invasive methods to detect pressure. The most widely accepted technique for animals is the tail-cuff method. iWorx offers the new IITC Non-Invasive Blood Pressure Systems for mice and rats. The IITC Life Science tail cuff system is a compact, integrated single unit which includes a warming chamber, restrainer and tail cuff. The system is controlled by a built in touch screen interface with controls for temperature, test cycle, cuff inflation, cuff calibration and various test parameters. Up to 200 systems can be daisy chained to record independently or simultaneously. Data can be downloaded directly onto a USB thumb drive while also being acquired via analog output by LabScribe software for further analysis. Various sizes of restrainers and tail cuffs are available to suit a variety of research models.
MRBP-MMouse Blood Pressure System (1 channel) MRBP-RRat Blood Pressure Systems (1 channel)
These systems are suitable for recording and measuring Respiratory Quotient, VO2 and VCO2 in small- to medium-size animals. The systems include the IX-Peak recorder and Gas Analyzer, LabScribe software with the Metabolic Calculations Module and either a 1.4 L or 5.2 L animal chamber.
iWorx IX-RA-834 8 Channel Recorder The iWorx RA-834 Recorder is the most advanced and feature-rich physiological data acquisition system on the market.
Allwood, Melissa A., Andrew J. Foster, Alicia M. Arkell, Marie-Soleil Beaudoin, Laelie A. Snook, Nadya Romanova, Coral L. Murrant, Graham P. Holloway, David C. Wright, and Jeremy A. Simpson. “Respiratory muscle weakness in the Zucker diabetic fatty rat.” American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2015): ajpregu-00447.